sialic acid (sa)  (GMP SA)

Journal: Experimental Animals

Article Title: Neu5Ac promotes high-fat diet-induced progression of atherosclerosis in ApoE -deficient mice

doi: 10.1538/expanim.25-0100

Figure Lengend Snippet: Neu5Ac levels and pro-atherogenic pathway activation in AS mice. (A) Bar charts of GO and KEGG enrichment analyses for key targets. (B) Neu5Ac levels in plasma and aortic lesion tissues, measured using a commercial assay kit, were significantly elevated in AS mice compared with WT controls. *** P <0.001.

Article Snippet: Plasma Neu5Ac levels were measured with a commercial colorimetric assay kit (E-BC-K068-M, Elabscience, Wuhan, China).

Techniques: Activation Assay, Clinical Proteomics

Journal: Experimental Animals

Article Title: Neu5Ac promotes high-fat diet-induced progression of atherosclerosis in ApoE -deficient mice

doi: 10.1538/expanim.25-0100

Figure Lengend Snippet: Neu5Ac disrupts serum lipid metabolism and promotes lipid deposition in AS mice. (A) Effects of Neu5Ac on serum lipid profiles: triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). (B) RT-qPCR and Western blot analyses demonstrated that Neu5Ac downregulated low-density lipoprotein receptor (LDLR) expression at both mRNA and protein levels. (C) Representative Oil Red O-stained aortic root sections illustrating lipid deposition across experimental groups (Scale bar=100 µ m). (D) Quantification of atherosclerotic lesion area. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Plasma Neu5Ac levels were measured with a commercial colorimetric assay kit (E-BC-K068-M, Elabscience, Wuhan, China).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining

Journal: Experimental Animals

Article Title: Neu5Ac promotes high-fat diet-induced progression of atherosclerosis in ApoE -deficient mice

doi: 10.1538/expanim.25-0100

Figure Lengend Snippet: Neu5Ac exacerbates atherosclerotic plaque necrosis in AS mice. (A) Representative H&E-stained aortic root sections illustrating necrotic core areas across experimental groups (Scale bar=100 µ m). (B) Quantitative analysis of necrotic core area. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Plasma Neu5Ac levels were measured with a commercial colorimetric assay kit (E-BC-K068-M, Elabscience, Wuhan, China).

Techniques: Staining

Journal: Experimental Animals

Article Title: Neu5Ac promotes high-fat diet-induced progression of atherosclerosis in ApoE -deficient mice

doi: 10.1538/expanim.25-0100

Figure Lengend Snippet: Neu5Ac enhances pro-inflammatory cytokine expression and oxidative stress in AS mice. (A) RT-qPCR analysis revealed significant upregulation of TNF-α , IL-6 , and MCP-1 mRNA expression in Neu5Ac-treated mice compared with AS mice. (B) Western blot analysis confirmed increased TNF-α, IL-6, and MCP-1 protein levels following Neu5Ac treatment. (C) Flow cytometry analysis demonstrated increased reactive oxygen species (ROS) production in the Neu5Ac group relative to the AS group. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Plasma Neu5Ac levels were measured with a commercial colorimetric assay kit (E-BC-K068-M, Elabscience, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry

Journal: Experimental Animals

Article Title: Neu5Ac promotes high-fat diet-induced progression of atherosclerosis in ApoE -deficient mice

doi: 10.1538/expanim.25-0100

Figure Lengend Snippet: Neu5Ac promotes a pro-inflammatory macrophage phenotype in AS mice. (A) Flow cytometry analysis demonstrated that Neu5Ac significantly increased the proportion of CD86 + M1 macrophages. (B) Neu5Ac treatment concurrently reduced the CD206 + M2 macrophage population compared with AS controls. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Plasma Neu5Ac levels were measured with a commercial colorimetric assay kit (E-BC-K068-M, Elabscience, Wuhan, China).

Techniques: Flow Cytometry